mouse anti-human polyclonal cdx2 antibody  (Biogenex)

Journal: The FASEB Journal

Article Title: The WNT signaling antagonist Dickkopf-1 directs lineage commitment and promotes survival of the preimplantation embryo

doi: 10.1096/fj.14-253112

Figure Lengend Snippet: Immunolocalization of CDX2 and GATA6 in blastocysts at d 8 of development. Hoechst 33342 was used as a nuclear stain for all blastomeres. Note the presence of Hoechst+ nuclei in the ICM that are negative for GATA6 (arrows) and Hoechst+ and GATA6+ nuclei in the edge of the ICM (arrowheads). Scale bars = 50 μm.

Article Snippet: Embryos were washed 6 times in wash buffer and incubated for 1 h with mouse anti-human polyclonal CDX2 antibody, ready to use (Biogenex, Fremont, CA, USA), followed by 6 washes in wash buffer and incubation with 1 μg/ml goat anti-mouse IgG conjugated with fluorescein isothiocyanate (FITC; Abcam, Cambridge, MA, USA) in the dark.

Techniques: Staining

Journal: The FASEB Journal

Article Title: The WNT signaling antagonist Dickkopf-1 directs lineage commitment and promotes survival of the preimplantation embryo

doi: 10.1096/fj.14-253112

Figure Lengend Snippet: Immunolocalization of GATA6 in blastocysts at d 7 and d 8 of development. A, B) Note that GATA6+ cells were widely distributed in both the ICM and TE at d 7. Arrows in panel B indicate cells clearly labeled for both GATA6 and CDX2. C, D) By d 8, labeling for GATA6 became more intense for ICM and less intense for TE and very few cells in the TE were labeled for GATA6. Scale bar = 50 μm.

Article Snippet: Embryos were washed 6 times in wash buffer and incubated for 1 h with mouse anti-human polyclonal CDX2 antibody, ready to use (Biogenex, Fremont, CA, USA), followed by 6 washes in wash buffer and incubation with 1 μg/ml goat anti-mouse IgG conjugated with fluorescein isothiocyanate (FITC; Abcam, Cambridge, MA, USA) in the dark.

Techniques: Labeling

Journal: The FASEB Journal

Article Title: The WNT signaling antagonist Dickkopf-1 directs lineage commitment and promotes survival of the preimplantation embryo

doi: 10.1096/fj.14-253112

Figure Lengend Snippet: Immunolocalization of NANOG, GATA6, and CDX2 in blastocysts at d 8 of development. NANOG+ cells were localized primarily in the center of the ICM, whereas GATA6+ cells were present primarily in the periphery of the ICM. Note the presence of faint GATA6 staining in the CDX2+ TE (arrows). Scale bars = 50 μm.

Article Snippet: Embryos were washed 6 times in wash buffer and incubated for 1 h with mouse anti-human polyclonal CDX2 antibody, ready to use (Biogenex, Fremont, CA, USA), followed by 6 washes in wash buffer and incubation with 1 μg/ml goat anti-mouse IgG conjugated with fluorescein isothiocyanate (FITC; Abcam, Cambridge, MA, USA) in the dark.

Techniques: Staining

Journal: The FASEB Journal

Article Title: The WNT signaling antagonist Dickkopf-1 directs lineage commitment and promotes survival of the preimplantation embryo

doi: 10.1096/fj.14-253112

Figure Lengend Snippet: Immunolocalization of GATA6, CDX2, and NANOG in d 8 blastocysts as visualized by confocal microscopy. A, B) Embryos labeled with GATA6 (red), CDX2 (green), and Hoechst (blue). In panel B, note that the TE has GATA6+ (arrowhead) as well as GATA6− cells (yellow arrow); also note the GATA6+ cell in the ICM, which represents a hypoblast cell (white arrow). C, D) Immunolocalization of GATA6 (red) and NANOG (green) in the ICM. D) Same embryo as in panel C but at higher magnification. Note that GATA6+ cells are in the periphery of the ICM, whereas NANOG+ cells are in the center of the ICM. Scale bar = 50 μm (A−C); scale bar = 16.7 μm (D).

Article Snippet: Embryos were washed 6 times in wash buffer and incubated for 1 h with mouse anti-human polyclonal CDX2 antibody, ready to use (Biogenex, Fremont, CA, USA), followed by 6 washes in wash buffer and incubation with 1 μg/ml goat anti-mouse IgG conjugated with fluorescein isothiocyanate (FITC; Abcam, Cambridge, MA, USA) in the dark.

Techniques: Confocal Microscopy, Labeling

Journal: The FASEB Journal

Article Title: The WNT signaling antagonist Dickkopf-1 directs lineage commitment and promotes survival of the preimplantation embryo

doi: 10.1096/fj.14-253112

Figure Lengend Snippet: DKK1 promotes lineage commitment. Results are least squares means ± sem for total cell number (A), percentage of blastomeres that were TE (CDX2+ cells; B) and ICM (CDX2− cells; C), TE/ICM ratio (D), percentage of blastomeres that were hypoblast (GATA6+ cells in ICM; E), and percentage that were GATA6+ in the TE (F) in blastocysts at d 7 and d 8. Treatment with DKK1 or vehicle started at d 5 of development. P values for individual tests appear on top of each graph when effects were significant. Trt, treatment.

Article Snippet: Embryos were washed 6 times in wash buffer and incubated for 1 h with mouse anti-human polyclonal CDX2 antibody, ready to use (Biogenex, Fremont, CA, USA), followed by 6 washes in wash buffer and incubation with 1 μg/ml goat anti-mouse IgG conjugated with fluorescein isothiocyanate (FITC; Abcam, Cambridge, MA, USA) in the dark.

Techniques: